Abstract
A hepatitis B virus preS2 deletion library with the preS2 sequence fused to the coat protein of the RNA phage fr (fr CP) as a carrier has been constructed and used for the approximate localization of epitope recognized by a panel of murine monoclonal anti-preS2 antibodies. DNA copies of putative preS2 epitopes were synthesized and cloned within the fr CP gene. Tetrapeptide Gln-Asp-Pro-Arg (QDPR) corresponding to the preS (132-135) sequence was found to be the minimal sufficient recognition site for one of the monoclonal antibodies, S26. The closely related tetrapeptide EDPR did not mimic the epitope activity of QDPR.
Original language | English |
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Pages (from-to) | 169-172 |
Number of pages | 4 |
Journal | Immunology Letters |
Volume | 33 |
Issue number | 2 |
DOIs | |
Publication status | Published - Jul 1992 |
Externally published | Yes |
Keywords*
- Anti-preS2 antibody
- Epitope
- PreS2 deletion library
Field of Science*
- 3.1 Basic medicine
- 1.6 Biological sciences
Publication Type*
- 1.1. Scientific article indexed in Web of Science and/or Scopus database