Tetrapeptide QDPR is a minimal immunodominant epitope within the preS2 domain of hepatitis B virus

I. Sominskaya; V. Bichko; P. Pushko; A. Dreimane; D. Snikere; P. Pumpens

doi:10.1016/0165-2478(92)90043-N

Tetrapeptide QDPR is a minimal immunodominant epitope within the preS2 domain of hepatitis B virus

I. Sominskaya
, V. Bichko
, P. Pushko
, A. Dreimane
, D. Snikere
, P. Pumpens

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A hepatitis B virus preS2 deletion library with the preS2 sequence fused to the coat protein of the RNA phage fr (fr CP) as a carrier has been constructed and used for the approximate localization of epitope recognized by a panel of murine monoclonal anti-preS2 antibodies. DNA copies of putative preS2 epitopes were synthesized and cloned within the fr CP gene. Tetrapeptide Gln-Asp-Pro-Arg (QDPR) corresponding to the preS (132-135) sequence was found to be the minimal sufficient recognition site for one of the monoclonal antibodies, S26. The closely related tetrapeptide EDPR did not mimic the epitope activity of QDPR.

Original language	English
Pages (from-to)	169-172
Number of pages	4
Journal	Immunology Letters
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(92)90043-N
Publication status	Published - Jul 1992
Externally published	Yes

Keywords*

Anti-preS2 antibody
Epitope
PreS2 deletion library

Field of Science*

3.1 Basic medicine
1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0165-2478(92)90043-N

https://www-sciencedirect-com.db.rsu.lv/science/article/pii/016524789290043N

Cite this

@article{0d5d89eb6e4942468a6fb3c59dca1ba3,

title = "Tetrapeptide QDPR is a minimal immunodominant epitope within the preS2 domain of hepatitis B virus",

abstract = "A hepatitis B virus preS2 deletion library with the preS2 sequence fused to the coat protein of the RNA phage fr (fr CP) as a carrier has been constructed and used for the approximate localization of epitope recognized by a panel of murine monoclonal anti-preS2 antibodies. DNA copies of putative preS2 epitopes were synthesized and cloned within the fr CP gene. Tetrapeptide Gln-Asp-Pro-Arg (QDPR) corresponding to the preS (132-135) sequence was found to be the minimal sufficient recognition site for one of the monoclonal antibodies, S26. The closely related tetrapeptide EDPR did not mimic the epitope activity of QDPR.",

keywords = "Anti-preS2 antibody, Epitope, PreS2 deletion library",

author = "I. Sominskaya and V. Bichko and P. Pushko and A. Dreimane and D. Snikere and P. Pumpens",

note = "Funding Information: This work was supported by grant No. 90.696 from the Latvian Council of Science.",

year = "1992",

month = jul,

doi = "10.1016/0165-2478(92)90043-N",

language = "English",

volume = "33",

pages = "169--172",

journal = "Immunology Letters",

issn = "0165-2478",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Tetrapeptide QDPR is a minimal immunodominant epitope within the preS2 domain of hepatitis B virus

AU - Sominskaya, I.

AU - Bichko, V.

AU - Pushko, P.

AU - Dreimane, A.

AU - Snikere, D.

AU - Pumpens, P.

N1 - Funding Information: This work was supported by grant No. 90.696 from the Latvian Council of Science.

PY - 1992/7

Y1 - 1992/7

N2 - A hepatitis B virus preS2 deletion library with the preS2 sequence fused to the coat protein of the RNA phage fr (fr CP) as a carrier has been constructed and used for the approximate localization of epitope recognized by a panel of murine monoclonal anti-preS2 antibodies. DNA copies of putative preS2 epitopes were synthesized and cloned within the fr CP gene. Tetrapeptide Gln-Asp-Pro-Arg (QDPR) corresponding to the preS (132-135) sequence was found to be the minimal sufficient recognition site for one of the monoclonal antibodies, S26. The closely related tetrapeptide EDPR did not mimic the epitope activity of QDPR.

AB - A hepatitis B virus preS2 deletion library with the preS2 sequence fused to the coat protein of the RNA phage fr (fr CP) as a carrier has been constructed and used for the approximate localization of epitope recognized by a panel of murine monoclonal anti-preS2 antibodies. DNA copies of putative preS2 epitopes were synthesized and cloned within the fr CP gene. Tetrapeptide Gln-Asp-Pro-Arg (QDPR) corresponding to the preS (132-135) sequence was found to be the minimal sufficient recognition site for one of the monoclonal antibodies, S26. The closely related tetrapeptide EDPR did not mimic the epitope activity of QDPR.

KW - Anti-preS2 antibody

KW - Epitope

KW - PreS2 deletion library

UR - https://www.scopus.com/pages/publications/0026650302

U2 - 10.1016/0165-2478(92)90043-N

DO - 10.1016/0165-2478(92)90043-N

M3 - Article

C2 - 1446923

AN - SCOPUS:0026650302

SN - 0165-2478

VL - 33

SP - 169

EP - 172

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

Tetrapeptide QDPR is a minimal immunodominant epitope within the preS2 domain of hepatitis B virus

Abstract

Keywords*

Field of Science*

Publication Type*

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this