The chloroplast protein BPG2 functions in brassinosteroid-mediated post-transcriptional accumulation of chloroplast rRNA

Tomoyuki Komatsu, Hiroshi Kawaide, Chieko Saito, Ayumi Yamagami, Setsuko Shimada, Miki Nakazawa, Minami Matsui, Akihiko Nakano, Masafumi Tsujimoto, Masahiro Natsume, Hiroshi Abe, Tadao Asami, Takeshi Nakano

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Brassinazole (Brz) is a specific inhibitor of the biosynthesis of brassinosteroids (BRs), which regulate plant organ and chloroplast development. We identified a recessive pale green Arabidopsis mutant, bpg2-1 (Brz-insensitive-pale green 2-1) that showed reduced sensitivity to chlorophyll accumulation promoted by Brz in the light. BPG2 encodes a chloroplast-localized protein with a zinc finger motif and four GTP-binding domains that are necessary for normal chloroplast biogenesis. BPG2-homologous genes are evolutionally conserved in plants, green algae and bacteria. Expression of BPG2 is induced by light and Brz. Chloroplasts of the bpg2-1 mutant have a decreased number of stacked grana thylakoids. In bpg2-1 and bpg2-2 mutants, there was no reduction in expression of rbcL and psbA, but there was abnormal accumulation of precursors of chloroplast 16S and 23S rRNA. Chloroplast protein accumulation induced by Brz was suppressed by the bpg2 mutation. These results indicate that BPG2 plays an important role in post-transcriptional and translational regulation in the chloroplast, and is a component of BR signaling.

Original languageEnglish
Pages (from-to)409-422
Number of pages14
JournalPlant Journal
Volume61
Issue number3
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Keywords

  • BR biosynthesis inhibitor Brz
  • Brassinosteroid
  • Chloroplast biogenesis
  • Chloroplast rRNA
  • GTPase
  • Processing

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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