Abstract
A composite scaffold of gelatine (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was successfully fabricated. Growth factors such as bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were loaded into the Gel-Pec-BCP hydrogel scaffolds by freeze-drying. The surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive and X-ray diffraction spectroscopy. The results obtained from Fourier transform infrared spectroscopy and quantitative measurements showed successfully loading of BMP-2 and VEGF into the Gel-Pec-BCP hydrogel scaffolds. In addition MC3T3-E1 preosteoblasts were cultivated on the three types of scaffolds to investigate the effects of BMP-2 and VEGF on cell viability and proliferation. The Gel-Pec-BCP scaffolds loaded with VEGF and BMP-2 demonstrated more cell spreading and proliferation compared to those of the Gel-Pec-BCP scaffolds.
| Original language | English |
|---|---|
| Article number | 41241 |
| Journal | Journal of Applied Polymer Science |
| Volume | 132 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 2015 |
| Externally published | Yes |
Keywords*
- Biocompatibility
- Biomaterials
- Porous materials
Field of Science*
- 1.4 Chemical sciences
- 1.6 Biological sciences
- 2.10 Nano-technology
- 2.5 Materials engineering
Publication Type*
- 1.1. Scientific article indexed in Web of Science and/or Scopus database