The effect of immunomodulation composition on systemic immune response and udder health in case of bovine subclinical mastitis

G. Gulbe, S. Doniņa, V. Saulīte, J. Jermolajevs, L. Peškova, Svirskis, A. Valdovska

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Modulation of mammary gland immune response may offer an alternative to antimicrobial therapy in the treatment of subclinical mastitis. The aim of the study is to investigate the systemic immunomodulating effect and the impact of composition LLG which consists of lysozyme, lactic acid and glycopeptides isolated from Lactobacillus spp. on udder health parameters. A total of 10 cows with subclinical mastitis were used in the study – 5 cows (19 udder quarters) in the experimental group and 5 other cows (19 udder quarters) in the control group. The experimental cows received intramammary infusions of LLG, given once per day, 3 times with the intervals of 48 h. Cows from the control group received infusions of sterile 0.15 M NaCl, given in the same way as the treated cows. The following parameters were measured: somatic cell count (SCC), the total number of blood leucocyte, differentiation between banded neutrophils, segmented neutrophils and lymphocytes, peripheral blood mononuclear cells and markers of cell activation. Besides bacteriological culturing was performed. Quarter milk and blood samples were taken several times from the 1st to the 21st day. During the treatment the number of lymphocytes and T helper cells significantly decreased in the blood of the treated group, the CD8+ cells did not change remarkably, the number of CD25+, CD38+, as well as CD69+ and CD95+ cells had diminished during the treatment. On the 21st day a rapid increase of IL-2 receptor bearing cells was detected. A significant elevation of SCC in the treated group was observed but pathogenic bacteria incidence decreased.

Original languageEnglish
Pages (from-to)144-154
Number of pages11
JournalAgronomy Research
Issue number1
Publication statusPublished - 2017


  • Immunomodulation
  • Lymphocyte subpopulations
  • Somatic cell count
  • Subclinical mastitis

Field of Science*

  • 4.3 Veterinary science
  • 4.2 Animal and Dairy science

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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