TY - JOUR
T1 - The evolutionary history and tissue mapping of amino acid transporters belonging to solute carrier families SLC32, SLC36, and SLC38
AU - Sundberg, Björn E.
AU - Wååg, Elin
AU - Jacobsson, Josefin A.
AU - Stephansson, Olga
AU - Rumaks, Juris
AU - Svirskis, Simons
AU - Alsiö, Johan
AU - Roman, Erika
AU - Ebendal, Ted
AU - Klusa, Vija
AU - Fredriksson, Robert
N1 - Funding Information:
Acknowledgment We would like to thank Dr. Helgi B. Schiöth for the valuable discussion during the course of the project. We would also like to thank Maria Hägglund for help with in situ hybridizations and Linnea Holmén for the preparation of Supplementary Fig. 1. R.F. was supported by the Swedish Brain Foundation (Hjärnfonden). E.R. was supported by the Swedish Brain Foundation (Hjärnfonden). The studies were supported by AFA, Svenska Läkaresällskapet, Lars Hiertas Foundation, O.E. och Edla Johanssons Foundation, Swedish Royal Academy of Sciences, Längmanska Kulturfonden, the Magnus Bergvall Foundation, and Åhlens Foundation.
PY - 2008/6
Y1 - 2008/6
N2 - Members of the solute carrier families (SLC) 32, 36, and 38, together also designated the β-group of SLCs, are known to transport neutral amino acids. In this paper, we show that these three families were present before the split of the animal lineage and that they are likely to share a common decent. We also show that the APF transporters found in plants are most likely homologous to the mammalian β-group, suggesting that this type of transporters arouse early in the evolution of eukaryotes. We performed detailed tissue expression analysis of all the members of the β-group in rat and found several examples of highly specific expression patterns, with SLC38A7 being exclusively found in liver, SLC38A5 in blood, and SLC38A4 in muscle and liver. Moreover, we found that SLC38A10 is expressed in several endocrine organs. We also found that SLC38A1 is highly up regulated in the cortex from rats treated with diazepam and that SLC38A2 is significantly down regulated in the same tissue. In addition, we performed a detailed expression analysis of SLC38A1 and SLC38A6 in mouse brain using in situ hybridization, which showed that both these transporters are widely expressed in the brain.
AB - Members of the solute carrier families (SLC) 32, 36, and 38, together also designated the β-group of SLCs, are known to transport neutral amino acids. In this paper, we show that these three families were present before the split of the animal lineage and that they are likely to share a common decent. We also show that the APF transporters found in plants are most likely homologous to the mammalian β-group, suggesting that this type of transporters arouse early in the evolution of eukaryotes. We performed detailed tissue expression analysis of all the members of the β-group in rat and found several examples of highly specific expression patterns, with SLC38A7 being exclusively found in liver, SLC38A5 in blood, and SLC38A4 in muscle and liver. Moreover, we found that SLC38A10 is expressed in several endocrine organs. We also found that SLC38A1 is highly up regulated in the cortex from rats treated with diazepam and that SLC38A2 is significantly down regulated in the same tissue. In addition, we performed a detailed expression analysis of SLC38A1 and SLC38A6 in mouse brain using in situ hybridization, which showed that both these transporters are widely expressed in the brain.
KW - Amino acid transporter
KW - Diazepam
KW - L-Dopa
KW - SLC38
KW - Solute carrier
UR - http://www.scopus.com/inward/record.url?scp=49849090426&partnerID=8YFLogxK
U2 - 10.1007/s12031-008-9046-x
DO - 10.1007/s12031-008-9046-x
M3 - Article
C2 - 18418736
AN - SCOPUS:49849090426
SN - 0895-8696
VL - 35
SP - 179
EP - 193
JO - Journal of Molecular Neuroscience
JF - Journal of Molecular Neuroscience
IS - 2
ER -