The FOX hunting system: An alternative gain-of-function gene hunting technique

Takanari Ichikawa, Miki Nakazawa, Mika Kawashima, Haruko Iizumi, Hirofumi Kuroda, Youichi Kondou, Yumi Tsuhara, Kumiko Suzuki, Akie Ishikawa, Motoaki Seki, Miki Fujita, Reiko Motohashi, Noriko Nagata, Tomoko Takagi, Kazuo Shinozaki, Minami Matsui

Research output: Contribution to journalArticlepeer-review

163 Citations (Scopus)

Abstract

We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T1 mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T2 progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.

Original languageEnglish
Pages (from-to)974-985
Number of pages12
JournalPlant Journal
Volume48
Issue number6
DOIs
Publication statusPublished - Dec 2006
Externally publishedYes

Keywords*

  • Arabidopsis
  • Dominant phenotype
  • Full-length cDNA
  • Functional genomics
  • Pale green
  • Unknown gene

Field of Science*

  • 1.6 Biological sciences

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

Fingerprint

Dive into the research topics of 'The FOX hunting system: An alternative gain-of-function gene hunting technique'. Together they form a unique fingerprint.

Cite this