The FOX hunting system: An alternative gain-of-function gene hunting technique

Takanari Ichikawa; Miki Nakazawa; Mika Kawashima; Haruko Iizumi; Hirofumi Kuroda; Youichi Kondou; Yumi Tsuhara; Kumiko Suzuki; Akie Ishikawa; Motoaki Seki; Miki Fujita; Reiko Motohashi; Noriko Nagata; Tomoko Takagi; Kazuo Shinozaki; Minami Matsui

doi:10.1111/j.1365-313X.2006.02924.x

The FOX hunting system: An alternative gain-of-function gene hunting technique

Takanari Ichikawa, Miki Nakazawa, Mika Kawashima, Haruko Iizumi, Hirofumi Kuroda, Youichi Kondou, Yumi Tsuhara, Kumiko Suzuki, Akie Ishikawa, Motoaki Seki, Miki Fujita, Reiko Motohashi, Noriko Nagata, Tomoko Takagi, Kazuo Shinozaki, Minami Matsui

Research output: Contribution to journal › Article › peer-review

187 Citations (Scopus)

Abstract

We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T₁ mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T₂ progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.

Original language	English
Pages (from-to)	974-985
Number of pages	12
Journal	Plant Journal
Volume	48
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-313X.2006.02924.x
Publication status	Published - Dec 2006
Externally published	Yes

Keywords*

Arabidopsis
Dominant phenotype
Full-length cDNA
Functional genomics
Pale green
Unknown gene

Field of Science*

1.6 Biological sciences

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1111/j.1365-313X.2006.02924.x

Cite this

Ichikawa, T., Nakazawa, M., Kawashima, M., Iizumi, H., Kuroda, H., Kondou, Y., Tsuhara, Y., Suzuki, K., Ishikawa, A., Seki, M., Fujita, M., Motohashi, R., Nagata, N., Takagi, T., Shinozaki, K., & Matsui, M. (2006). The FOX hunting system: An alternative gain-of-function gene hunting technique. Plant Journal, 48(6), 974-985. https://doi.org/10.1111/j.1365-313X.2006.02924.x

@article{d82399d0f8ef4868bd12d411946b9502,

title = "The FOX hunting system: An alternative gain-of-function gene hunting technique",

abstract = "We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T1 mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T2 progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.",

keywords = "Arabidopsis, Dominant phenotype, Full-length cDNA, Functional genomics, Pale green, Unknown gene",

author = "Takanari Ichikawa and Miki Nakazawa and Mika Kawashima and Haruko Iizumi and Hirofumi Kuroda and Youichi Kondou and Yumi Tsuhara and Kumiko Suzuki and Akie Ishikawa and Motoaki Seki and Miki Fujita and Reiko Motohashi and Noriko Nagata and Tomoko Takagi and Kazuo Shinozaki and Minami Matsui",

year = "2006",

month = dec,

doi = "10.1111/j.1365-313X.2006.02924.x",

language = "English",

volume = "48",

pages = "974--985",

journal = "Plant Journal",

issn = "0960-7412",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "6",

}

Ichikawa, T, Nakazawa, M, Kawashima, M, Iizumi, H, Kuroda, H, Kondou, Y, Tsuhara, Y, Suzuki, K, Ishikawa, A, Seki, M, Fujita, M, Motohashi, R, Nagata, N, Takagi, T, Shinozaki, K & Matsui, M 2006, 'The FOX hunting system: An alternative gain-of-function gene hunting technique', Plant Journal, vol. 48, no. 6, pp. 974-985. https://doi.org/10.1111/j.1365-313X.2006.02924.x

TY - JOUR

T1 - The FOX hunting system

T2 - An alternative gain-of-function gene hunting technique

AU - Ichikawa, Takanari

AU - Nakazawa, Miki

AU - Kawashima, Mika

AU - Iizumi, Haruko

AU - Kuroda, Hirofumi

AU - Kondou, Youichi

AU - Tsuhara, Yumi

AU - Suzuki, Kumiko

AU - Ishikawa, Akie

AU - Seki, Motoaki

AU - Fujita, Miki

AU - Motohashi, Reiko

AU - Nagata, Noriko

AU - Takagi, Tomoko

AU - Shinozaki, Kazuo

AU - Matsui, Minami

PY - 2006/12

Y1 - 2006/12

N2 - We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T1 mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T2 progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.

AB - We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T1 mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T2 progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs.

KW - Arabidopsis

KW - Dominant phenotype

KW - Full-length cDNA

KW - Functional genomics

KW - Pale green

KW - Unknown gene

UR - http://www.scopus.com/inward/record.url?scp=33845523291&partnerID=8YFLogxK

U2 - 10.1111/j.1365-313X.2006.02924.x

DO - 10.1111/j.1365-313X.2006.02924.x

M3 - Article

C2 - 17227551

AN - SCOPUS:33845523291

SN - 0960-7412

VL - 48

SP - 974

EP - 985

JO - Plant Journal

JF - Plant Journal

IS - 6

ER -

The FOX hunting system: An alternative gain-of-function gene hunting technique

Abstract

Keywords*

Field of Science*

Publication Type*

Access to Document

Other files and links

Fingerprint

Cite this