TY - JOUR
T1 - The Gut Microbiome among Postmenopausal Latvian Women in Relation to Dietary Habits
AU - Aumeistere, Līva
AU - Ķibilds, Juris
AU - Siksna, Inese
AU - Neimane, Lolita Vija
AU - Kampara, Māra
AU - Ļubina, Olga
AU - Ciproviča, Inga
N1 - Funding Information:
This research was funded by the Project “Scientifically substantiated fermented milk products development and their clinical studies”, grant number 19-00-A01612-000009, financed by European agricultural fund for rural development.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/8/30
Y1 - 2022/8/30
N2 - In recent years, many studies have been initiated to characterise the human gut microbiome in relation to different factors like age, lifestyle, and dietary habits. This study aimed to evaluate the impact of yoghurt intake on the gut microbiome among postmenopausal women and how overall dietary habits modulate the gut microbiome. In total, 52 participants were included in the study and two groups—a control (n = 26) and experimental group (n = 26)—were established. The study was eight weeks long. Both study groups were allowed to consume a self-selected diet, but the experimental group had to additionally consume 175 g of plain organic milk yoghurt on a daily basis for eight weeks. In addition, a series of questionnaires were completed, including a questionnaire on the subject’s sociodemographic background, health status, and lifestyle factors, as well as a food frequency questionnaire. Stool samples were collected for the analysis of the gut microbiome (both prior to and after the eight weeks of the study). Sequencing of V4-V5 regions of the 16S rRNA gene was used to determine the bacterial composition of stool samples. The dominant phylum from the gut microbiome was Firmicutes (~70% to 73%), followed by Bacteroidota (~20% to 23%). Although no significant changes in the gut microbiome were related to daily consumption of yoghurt, we report that consumption of food products like grains, grain-based products, milk and milk products, and beverages (tea, coffee) is associated with differences in the composition of the gut microbiome. Establishing nutritional strategies to shape the gut microbiome could contribute to improved health status in postmenopausal women, but further research is needed.
AB - In recent years, many studies have been initiated to characterise the human gut microbiome in relation to different factors like age, lifestyle, and dietary habits. This study aimed to evaluate the impact of yoghurt intake on the gut microbiome among postmenopausal women and how overall dietary habits modulate the gut microbiome. In total, 52 participants were included in the study and two groups—a control (n = 26) and experimental group (n = 26)—were established. The study was eight weeks long. Both study groups were allowed to consume a self-selected diet, but the experimental group had to additionally consume 175 g of plain organic milk yoghurt on a daily basis for eight weeks. In addition, a series of questionnaires were completed, including a questionnaire on the subject’s sociodemographic background, health status, and lifestyle factors, as well as a food frequency questionnaire. Stool samples were collected for the analysis of the gut microbiome (both prior to and after the eight weeks of the study). Sequencing of V4-V5 regions of the 16S rRNA gene was used to determine the bacterial composition of stool samples. The dominant phylum from the gut microbiome was Firmicutes (~70% to 73%), followed by Bacteroidota (~20% to 23%). Although no significant changes in the gut microbiome were related to daily consumption of yoghurt, we report that consumption of food products like grains, grain-based products, milk and milk products, and beverages (tea, coffee) is associated with differences in the composition of the gut microbiome. Establishing nutritional strategies to shape the gut microbiome could contribute to improved health status in postmenopausal women, but further research is needed.
KW - Gut microbiome
KW - Yoghurt
KW - Dietary habits
KW - Postmenopausal period
UR - http://www.scopus.com/inward/record.url?scp=85137555431&partnerID=8YFLogxK
U2 - 10.3390/nu14173568
DO - 10.3390/nu14173568
M3 - Article
C2 - 36079824
SN - 2072-6643
VL - 14
SP - 1
EP - 13
JO - Nutrients
JF - Nutrients
IS - 17
M1 - 3568
ER -