The influence of cultivation conditions on the proliferation and differentiation of rat bone marrow multipotent mesenchymal stromal cells

Gita Kievina, N. Bezborodovs, G. Makarenkova, S. Nikulsins, Z. Krumina, D. Babarikins

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

Rat bone marrow multipotent mesenchymal stromal cells (rBM MMSCs) demonstrate a potential to differentiate down the mesodermal pathway but under appropriate experimental condition differentiation into non-mesodermal lineages may occur. Knowing this fact it seems interesting whether prolonged cultivation and preservation affects BM MMSC morphology, differentiation ability into multiple lineages and proliferation capacity. Therefore this study was aimed to investigate the effects of long-term cultivation and cryopreservation on the morphology, proliferation and differentiation potential of rat bone marrow-derived MMSCs in vitro. The effects of long term cultivation and cryopreservation were evaluated by the phenotype and morphology of the cell population. Findings show that the 60th passage cells had a smaller, more flattened and spindle-shaped morphology than the 4th passage cells before and after cryopreservation. We have also found that the 60 th passage cells were more proliferative than rBM MMSCs in early passages but the ability to differentiate was more effective in the 4 th passage cells before cryopreservation. rBM MMSCs from pre- and post- cryopreservation had similar colony formation potential and cellular morphology but disparity in differentiation potential was observed. The results of the present study provide evidence that rBM MMSCs could be cryopreserved without noticeable loss of viability and proliferative capacity but the differentiation capability may be affected.

Original languageEnglish
Title of host publication14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics
Editors Alexei Katashev, Yuri Dekhtyar, Janis Spigulis
PublisherSpringer
Pages41-44
Number of pages4
ISBN (Print)9783540693666
DOIs
Publication statusPublished - 2008
Event14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics - Riga, Latvia
Duration: 16 Jun 200820 Jun 2008
Conference number: 14

Publication series

NameIFMBE Proceedings
Volume20 IFMBE
ISSN (Print)1680-0737

Conference

Conference14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics
Abbreviated titleNBC 2008
Country/TerritoryLatvia
CityRiga
Period16/06/0820/06/08

Keywords

  • Bone marrow multipotent mesenchymal stromal cells
  • cryopreservation
  • long-term culture
  • transdifferentiation

Field of Science

  • 2.4 Chemical engineering
  • 1.6 Biological sciences

Publication Type

  • 3.1. Articles or chapters in proceedings/scientific books indexed in Web of Science and/or Scopus database

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