TY - JOUR
T1 - Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury
AU - Williams, Jonathan A.
AU - Huesa, Carmen
AU - Turunen, Mikael J.
AU - Oo, James A.
AU - Radzins, Oskars
AU - Gardner, Wilf
AU - Windmill, James F.C.
AU - Isaksson, Hanna
AU - Tanner, K. Elizabeth
AU - Riddell, John S.
AU - Coupaud, Sylvie
N1 - Funding Information:
This study was supported by funding from the Engineering and Physical Sciences Research Council (EPSRC) Life Sciences Interface Programme (EP/F50036X/1) and in part by the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 615030. Carmen Huesa was supported by the Versus Arthritis early career fellowship (grant no. 22483). Data collected in this study is accessible at https://doi. org/10.15129/e345cd3a-e306-4ceb-b1c3-616da9ec40bd.
Publisher Copyright:
© 2022, International Society of Musculoskeletal and Neuronal Interactions. All rights reserved.
PY - 2022/6
Y1 - 2022/6
N2 - Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10-or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy. Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6-and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.
AB - Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10-or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy. Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6-and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.
KW - Bone Mechanical Properties
KW - Fourier-transform Infrared Spectroscopy
KW - microCT
KW - Osteoporosis
KW - Spinal Cord Transection
UR - http://www.scopus.com/inward/record.url?scp=85131091955&partnerID=8YFLogxK
M3 - Article
C2 - 35642701
AN - SCOPUS:85131091955
SN - 1108-7161
VL - 22
SP - 212
EP - 234
JO - Journal of Musculoskeletal Neuronal Interactions
JF - Journal of Musculoskeletal Neuronal Interactions
IS - 2
ER -
