Transcription of HERV-E and HERV-E-related sequences in malignant and non-malignant human haematopoietic cells

Bhupesh K Prusty; Harald zur Hausen; Rainer Schmidt; Romana Kimmel; Ethel-Michele de Villiers

doi:10.1016/j.virol.2008.09.006

Transcription of HERV-E and HERV-E-related sequences in malignant and non-malignant human haematopoietic cells

Bhupesh K Prusty, Harald zur Hausen, Rainer Schmidt, Romana Kimmel, Ethel-Michele de Villiers (Corresponding Author)

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Infection of a human T-cell leukaemia cell line (HSB-2) with HHV-6 led to the induction of exosome-like-particles attached to newly formed HHV-6 enveloped particles and to amplification of a 1642 bp molecule consisting of a partial human endogenous retrovirus (HERV)-E polymerase gene and repetitive sequences. We initiated an analysis of transcriptional patterns of predicted genes from HERV-E sequences in normal and malignant haematopoietic cells. Transcription patterns of regions corresponding to gag, pol and env genes at different chromosomal loci varied among cell types tested. Several specific transcripts were only observed in malignant haematopoietic cells and transcriptional activity varied among different malignant cell types. A transcript of 7.1 kb spanning the complete gag, pol and env gene region, originating from chromosome 8p23, was identified in normal peripheral blood cells and cells of the chronic myeloid leukaemia cell line K562. Our study describes new active HERV-E sequences and new loci throughout the human genome.

Original language	English
Pages (from-to)	37-45
Number of pages	9
Journal	Virology
Volume	382
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2008.09.006
Publication status	Published - 5 Dec 2008
Externally published	Yes

Keywords*

Blood Cells/virology
Cell Line
Cell Line, Tumor
Endogenous Retroviruses/genetics
Gene Expression Profiling
Genes, Viral
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/virology
Transcription, Genetic

Field of Science*

3.1 Basic medicine
3.2 Clinical medicine

Publication Type*

1.1. Scientific article indexed in Web of Science and/or Scopus database

Access to Document

10.1016/j.virol.2008.09.006

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title = "Transcription of HERV-E and HERV-E-related sequences in malignant and non-malignant human haematopoietic cells",

abstract = "Infection of a human T-cell leukaemia cell line (HSB-2) with HHV-6 led to the induction of exosome-like-particles attached to newly formed HHV-6 enveloped particles and to amplification of a 1642 bp molecule consisting of a partial human endogenous retrovirus (HERV)-E polymerase gene and repetitive sequences. We initiated an analysis of transcriptional patterns of predicted genes from HERV-E sequences in normal and malignant haematopoietic cells. Transcription patterns of regions corresponding to gag, pol and env genes at different chromosomal loci varied among cell types tested. Several specific transcripts were only observed in malignant haematopoietic cells and transcriptional activity varied among different malignant cell types. A transcript of 7.1 kb spanning the complete gag, pol and env gene region, originating from chromosome 8p23, was identified in normal peripheral blood cells and cells of the chronic myeloid leukaemia cell line K562. Our study describes new active HERV-E sequences and new loci throughout the human genome.",

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AU - Prusty, Bhupesh K

AU - zur Hausen, Harald

AU - Schmidt, Rainer

AU - Kimmel, Romana

AU - de Villiers, Ethel-Michele

PY - 2008/12/5

Y1 - 2008/12/5

N2 - Infection of a human T-cell leukaemia cell line (HSB-2) with HHV-6 led to the induction of exosome-like-particles attached to newly formed HHV-6 enveloped particles and to amplification of a 1642 bp molecule consisting of a partial human endogenous retrovirus (HERV)-E polymerase gene and repetitive sequences. We initiated an analysis of transcriptional patterns of predicted genes from HERV-E sequences in normal and malignant haematopoietic cells. Transcription patterns of regions corresponding to gag, pol and env genes at different chromosomal loci varied among cell types tested. Several specific transcripts were only observed in malignant haematopoietic cells and transcriptional activity varied among different malignant cell types. A transcript of 7.1 kb spanning the complete gag, pol and env gene region, originating from chromosome 8p23, was identified in normal peripheral blood cells and cells of the chronic myeloid leukaemia cell line K562. Our study describes new active HERV-E sequences and new loci throughout the human genome.

AB - Infection of a human T-cell leukaemia cell line (HSB-2) with HHV-6 led to the induction of exosome-like-particles attached to newly formed HHV-6 enveloped particles and to amplification of a 1642 bp molecule consisting of a partial human endogenous retrovirus (HERV)-E polymerase gene and repetitive sequences. We initiated an analysis of transcriptional patterns of predicted genes from HERV-E sequences in normal and malignant haematopoietic cells. Transcription patterns of regions corresponding to gag, pol and env genes at different chromosomal loci varied among cell types tested. Several specific transcripts were only observed in malignant haematopoietic cells and transcriptional activity varied among different malignant cell types. A transcript of 7.1 kb spanning the complete gag, pol and env gene region, originating from chromosome 8p23, was identified in normal peripheral blood cells and cells of the chronic myeloid leukaemia cell line K562. Our study describes new active HERV-E sequences and new loci throughout the human genome.

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KW - Endogenous Retroviruses/genetics

KW - Gene Expression Profiling

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KW - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/virology

KW - Transcription, Genetic

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Transcription of HERV-E and HERV-E-related sequences in malignant and non-malignant human haematopoietic cells

Abstract

Keywords*

Field of Science*

Publication Type*

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