Troubleshooting digital macro photography for image acquisition and the analysis of biological samples

Edgars Liepinsh, Janis Kuka, Maija Dambrova

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


For years, image acquisition and analysis have been an important part of life science experiments to ensure the adequate and reliable presentation of research results. Since the development of digital photography and digital planimetric methods for image analysis approximately 20. years ago, new equipment and technologies have emerged, which have increased the quality of image acquisition and analysis. Different techniques are available to measure the size of stained tissue samples in experimental animal models of disease; however, the most accurate method is digital macro photography with software that is based on planimetric analysis. In this study, we described the methodology for the preparation of infarcted rat heart and brain tissue samples before image acquisition, digital macro photography techniques and planimetric image analysis. These methods are useful in the macro photography of biological samples and subsequent image analysis. In addition, the techniques that are described in this study include the automated analysis of digital photographs to minimize user input and exclude the risk of researcher-generated errors or bias during image analysis.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalJournal of Pharmacological and Toxicological Methods
Issue number2
Publication statusPublished - Mar 2013
Externally publishedYes


  • Brain infarction
  • Imaging
  • Myocardial infarction
  • Planimetric analysis
  • Sample preparation
  • TTC (2,3,5-triphenyltetrazolium chloride)

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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