TY - JOUR
T1 - Uspostavljanje referentnih intervala za multimere faktora von Willebrand
AU - Pikta, Marika
AU - Vasse, Marc
AU - Smock, Kristi J.
AU - Moser, Karen A.
AU - van Dievoet, Marie Astrid
AU - Lejniece, Sandra
AU - Szanto, Timea
AU - Bautista, Hector
AU - Nouadje, George
AU - Banys, Valdas
N1 - Funding Information:
We are grateful to Sebia (France) for the funding of this project. We would like to thank all the medical/laboratory staff and the volunteers who contributed to this study. The preliminary results of this study were discussed at IFCC C-RIDL (Scientific Committee of IFCC on Reference Intervals and Decision Limits) closed meeting during Euromedlab2019 Barcelona congress and presented at the ISTH 2020 Virtual Congress (abstract/e-poster).
Publisher Copyright:
© 2022 Sciendo. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.
AB - Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.
KW - Quantitative analysis
KW - Reference intervals
KW - Von Willebrand factor
KW - Von Willebrand factor multimers
UR - http://www.scopus.com/inward/record.url?scp=85124964840&partnerID=8YFLogxK
U2 - 10.5937/JOMB0-31941
DO - 10.5937/JOMB0-31941
M3 - Article
AN - SCOPUS:85124964840
SN - 1452-8258
VL - 41
SP - 115
EP - 121
JO - Journal of Medical Biochemistry
JF - Journal of Medical Biochemistry
IS - 1
ER -