Uspostavljanje referentnih intervala za multimere faktora von Willebrand

Translated title of the contribution: Establishing reference intervals for von Willebrand factor multimers

Marika Pikta, Marc Vasse, Kristi J. Smock, Karen A. Moser, Marie Astrid van Dievoet, Sandra Lejniece, Timea Szanto, Hector Bautista, George Nouadje, Valdas Banys

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
3 Downloads (Pure)


Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.

Translated title of the contributionEstablishing reference intervals for von Willebrand factor multimers
Original languageBosnian
Pages (from-to)115-121
Number of pages7
JournalJournal of Medical Biochemistry
Issue number1
Publication statusPublished - 2022


  • Quantitative analysis
  • Reference intervals
  • Von Willebrand factor
  • Von Willebrand factor multimers

Field of Science*

  • 3.1 Basic medicine

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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