Viscoll collagen solution as a novel bioink for direct 3D bioprinting

Egor O. Osidak, Pavel A. Karalkin, Maria S. Osidak, Vladislav A. Parfenov, Dmitriy E. Sivogrivov, Frederico D.A.S. Pereira, Anna A. Gryadunova, Elizaveta V. Koudan, Yusef D. Khesuani, Vladimir Kasyanov, Sergei I. Belousov, Sergey V. Krasheninnikov, Timofei E. Grigoriev, Sergey N. Chvalun, Elena A. Bulanova, Vladimir A. Mironov, Sergey P. Domogatsky

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)


Collagen is one of the most promising materials for 3D bioprinting because of its distinguished biocompatibility. Cell-laden constructs made of pure collagen with or without incorporated growth supplements support engineered constructs persistence in culture and are perfectly suitable for grafting. The limiting factor for direct 3D collagen printing was poor printability of collagen solutions, especially admixed with cells or tissue spheroids. In our study, we showed that concentrated solutions of native collagen branded Viscoll were effective as bioinks with high fidelity performance. Viscoll containing 20, 30, or 40 mg/ml collagen were used for direct extrusion 3D bioprinting to form scaffolds appropriate to support spatial arrangement of tissue spheroids into rigid patterns with resolution of 0.5 mm in details. Incorporated cells demonstrated sufficient viability. Associated rheological study showed that good printability of the collagen solutions correlates with their increased storage modulus value, notably exceeding the loss modulus value. The proper combination of these physical parameters could become technological criteria for manufacturing various collagen bioinks for 3D bioprinting. [Figure not available: see fulltext.].

Original languageEnglish
Article number31
Number of pages12
JournalJournal of Materials Science: Materials in Medicine
Issue number3
Publication statusPublished - 1 Mar 2019

Field of Science*

  • 2.5 Materials engineering
  • 2.6 Medical engineering

Publication Type*

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database


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