ydk1-D, an auxin-responsive GH3 mutant that is involved in hypocotyl and root elongation

Tomoyuki Takase, Miki Nakazawa, Akie Ishikawa, Mika Kawashima, Takanari Ichikawa, Naoki Takahashi, Hiroaki Shimada, Katsushi Manabe, Minami Matsui

Research output: Contribution to journalArticlepeer-review

136 Citations (Scopus)

Abstract

To study the GH3 gene family of Arabidopsis, we investigated a flanking sequence database of Arabidopsis activation-tagged lines. We found a dwarf mutant, named yadokari 1-D (ydk1-D), that had a T-DNA insertion proximal to a GH3 gene. ydk1-D is dominant and has a short hypocotyl not only in light but also in darkness. Moreover, ydk1-D has a short primary root, a reduced lateral root number, and reduced apical dominance. A GH3 gene, named YDK1, was upregulated in ydk1-D, and YDK1 transgenic plants showed the ydk1-D phenotype. YDK1 gene expression was induced by exogenously applied auxin and regulated by auxin-response factor (ARF)7. In addition, YDK1 gene expression was downregulated by blue and far-red (FR) lights. Strong promoter activity of YDK1 was observed in roots and flowers. These results suggest that YDK1 may function as a negative component in auxin signaling by regulating auxin activity.

Original languageEnglish
Pages (from-to)471-483
Number of pages13
JournalPlant Journal
Volume37
Issue number4
DOIs
Publication statusPublished - Feb 2004
Externally publishedYes

Keywords

  • Arabidopsis
  • Auxin
  • GH3
  • Hypocotyl elongation
  • Light
  • Root elongation

Field of Science

  • 1.6 Biological sciences

Publication Type

  • 1.1. Scientific article indexed in Web of Science and/or Scopus database

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